Single-base mismatch recognition using partially double-stranded probes having various lengths
نویسندگان
چکیده
منابع مشابه
Mixed-Sequence Recognition of Double-Stranded DNA Using Enzymatically Stable Phosphorothioate Invader Probes.
Development of probes that allow for sequence-unrestricted recognition of double-stranded DNA (dsDNA) continues to attract much attention due to the prospect for molecular tools that enable detection, regulation, and manipulation of genes. We have recently introduced so-called Invader probes as alternatives to more established approaches such as triplex-forming oligonucleotides, peptide nucleic...
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The development of molecular strategies that enable recognition of specific double-stranded DNA (dsDNA) regions has been a longstanding goal as evidenced by the emergence of triplex-forming oligonucleotides, peptide nucleic acids (PNAs), minor groove binding polyamides, and--more recently--engineered proteins such as CRISPR/Cas9. Despite this progress, an unmet need remains for simple hybridiza...
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Within the preprocessing pipeline of a Next Generation Sequencing sample, its set of Single-Base Mismatches is one of the first outcomes, together with the number of correctly aligned reads. The union of these two sets provides a 4× 4 matrix (called Single Base Indicator, SBI in what follows) representing a blueprint of the sample and its preprocessing ingredients such as the sequencer, the ali...
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ژورنال
عنوان ژورنال: Nucleic Acids Symposium Series
سال: 2006
ISSN: 1746-8272,0261-3166
DOI: 10.1093/nass/nrl162